Spectrophotometric micromethod for measuring cholinesterase activity in serum or plasma.
نویسندگان
چکیده
A simple ultraviolet method has been developed for determining serum or plasma cholinesterase activity. The cholinesterase acts on acetylor butyrylthiocholine to release thiocholine, which reacts with 2,2’or 4,4’-dithiodipyridine, showing absorbance at 324 or 343 nm, respectively. The activity can be measured kinetically and alsoafter incubation for 3 to 5 mm at 20#{176} to 37#{176}C. A volume of 10l of serum or plasma is sufficient for the determination. This method is well suited for clinical investigation as well as experimental studies.
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عنوان ژورنال:
- Clinical chemistry
دوره 18 5 شماره
صفحات -
تاریخ انتشار 1972